The protection of yupingfeng powder on cisplatin induced oxidative damage of organs in hepatocellular carcinoma mice / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To study the effects of Yupingfeng Powder (YPFP) on cisplatin (DDP) induced oxidative damage of organs in hepatocellular carcinoma mice.</p><p><b>METHODS</b>A total of 2 x10(6) Hepa1 -6 cells were inoculated subcutaneously into the right flank of 15 C57BL/6 mice to establish a mice model of hepatocellular carcinoma. Then the mice were randomly divided into three groups, i.e., the model group, the DDP group, and the DDP + YPFP group, 5 in each group. Mice in the DDP group and the DDP + YPFP group were intraperitoneally injected with DDP (2. 5 mg/kg), once every three day for 2 weeks. Physiological saline was intraperitoneally injected to mice in the model group. Meanwhile, YPFP water decoction (25 g/kg) was given to mice in the DDP + YPFP group by gastrogavage once daily for 2 weeks. Corresponding distilled water was given by gastrogavage to mice in the DDP group and the model group. Fourteen days later, mice were sacrificed and the tumor inhibition ratio was calculated. The weights of kidneys, livers, and lungs were weighed and the organ coefficient calculated. The activities of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in the tissue were detected. The pathologic changes were observed.</p><p><b>RESULTS</b>The tumor weight obviously decreased in the DDP group and the DDP + YPFP group when compared with the model group (P < 0.05, P < 0.01). Obvious oxidative damage existed in the kidneys and livers after induced by DDP. Oxidative damage also existed in the lungs to some extent. YPFP could obviously decrease the content of MDA and the activities of SOD in livers (P < 0.05), and increase the activities of SOD in lungs (P < 0.01). The pathologic changes showed the same effect trend.</p><p><b>CONCLUSIONS</b>YPFP could protect the organs (kidney, liver, lung) from the oxidative damage induced by DDP. Anti-oxidation is one of its mechanisms.</p>

Expression of PH20 in primary and metastatic breast cancer and its pathological significance / 中华病理学杂志

<p><b>OBJECTIVE</b>To evaluate the expression pattern of PH20 in primary and metastatic breast cancer and its relationship to tumor metastatic potential.</p><p><b>METHODS</b>Anti-PH20 antibody was synthesized by injection of conjugated human PH20 peptides into rabbits. Immunohistochemical study was performed on 53 cases of human breast cancer. Western blot was used to detect PH20 expression in 5 cases of breast cancer with available fresh tissue. Two oligonucleotide probes were prepared for in-situ hybridization using breast tissue microarray.</p><p><b>RESULTS</b>Normal breast tissue did not express PH20 (0/3), while 58.4% (31/53) of breast cancer cases did. The highest expression rate was found in metastatic foci in regional lymph nodes (83.3%), followed by primary breast cancer tissue in cases with lymph node secondaries (70.8%). The breast cancer cases with no any metastasis had an expression rate of 48.2%. The immunohistochemical staining results were further confirmed by Western blotting. In-situ hybridization showed PH20 RNA in 75% of the breast cancer tissue (21/28). Two of the 17 cases of normal breast tissue showed weak expression in some ductolobular units.</p><p><b>CONCLUSIONS</b>The expression of PH20 has a positive correlation with metastatic potential in breast cancer. It is possible that PH20 may play an important role in the invasive growth and metastasis of breast cancer cells, via mechanisms such as digestion of surrounding stromal tissue and release of FGF-2.</p>

Metabolism of hyaluronic acid and extremity lymphedema / 中华整形外科杂志

<p><b>OBJECTIVE</b>To investigate the possible influence of the impairment of lymph fluid on the metabolism of hyaluronic acid (HA) in the lymphedematous skin tissue.</p><p><b>METHODS</b>Tissue fluid was collected in lymphedematous limbs and the contralateral healthy limbs of 39 patients and HA content was measured with radioimmunoassay. The protein contents were also measured.</p><p><b>RESULTS</b>The HA contents in interstitial fluid of lymphedematous limb were significantly (8 fold) higher than that of normal limb. The protein concentration in the tissue fluid did not show significant differences between lymphedema and those with normal tissue.</p><p><b>CONCLUSION</b>The result suggests blockage of regional draining lymphatics may impairs breakdown of HA and the stagnation of HA in the limb may exert a deleterious effect on the interstitium.</p>